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Oxford Agar, Modified (Listeria Selective Agar, Modified Oxford) (MOX Agar)

[所属分类:培养基配方] [发布时间:2021-12-8] [发布人:网站管理员2] [阅读次数:] [返回]

Oxford Agar, Modified

(Listeria Selective Agar,

Modified Oxford)

(MOX Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Special peptone.................................................23.0g

LiCl ...................................................................15.0g

Agar ..................................................................12.0g

NaCl....................................................................5.0g

Cornstarch...........................................................1.0g

Esculin ................................................................1.0g

Ferric ammonium citrate.....................................0.5g

Antibiotic inhibitor ....................................... 10.0mL

pH 7.0 ± 0.2 at 25°C

Antibiotic Inhibitor:

Composition per 10.0mL:

Moxalactam ....................................................0.015g

Colistin sulfate ..................................................0.01g

Preparation of Antibiotic Inhibitor: Add com

ponents to distilled /deionized water and bring vol

ume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept antibiotic inhibitor, to distilled/deionized water

and bring volume to 990.0mL. Mix thoroughly. Gen

tly heat and bring to boiling. Autoclave for 10 min at

15 psi pressure–121°C. Cool to 45°–50°C. Aseptical

ly add 10.0mL of sterile antibiotic inhibitor. Mix

thoroughly. Pour into sterile Petri dishes or distribute

into sterile tubes.

Use: For the isolation and cultivation of Listeria

monocytogenes from specimens containing a mixed

bacterial flora.