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Ogawa TB Medium

[所属分类:培养基配方] [发布时间:2021-12-7] [发布人:网站管理员2] [阅读次数:] [返回]

Ogawa TB Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 300.0mL:

KH2PO4...............................................................1.0g

Homogenized whole egg ............................ 200.0mL

Glycerol .......................................................... 6.0mL

Malachite Green (2% solution)....................... 6.0mL

pH 6.5 ± 0.2 at 25°C

Homogenized Whole Egg:

Composition per liter:

Whole eggs ......................................................18–24

Preparation of Homogenized Whole Egg:

Use fresh eggs, less than 1 week old. Scrub the shells

with soap. Let stand in a soap solution for 30 min.

Rinse in running water. Soak eggs in 70% ethanol for

15 min. Break the eggs into a sterile container. Ho

mogenize by shaking. Filter through four layers of

sterile cheesecloth into a sterile graduated cylinder.

Measure out 1.0L.

Preparation of Medium: Add components, ex

cept homogenized whole egg, to distilled/deionized

water and bring volume to 100.0mL. Mix thoroughly.

Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 45°–50°C. Aseptically add 200.0mL of sterile ho

mogenized whole egg. Mix thoroughly. Aseptically

distribute into sterile screw-capped tubes in 7.0mL

volumes. Inspissate at 85°–90°C (moist heat) for 60

min.

Use: For the isolation and cultivation of Mycobacte

rium species, except for Mycobacterium leprae.