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VL Medium

[所属分类:培养基配方] [发布时间:2021-10-11] [发布人:网站管理员2] [阅读次数:] [返回]

VL Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 10.0g

Agar .............................................................................................. 6.0g

NaCl.............................................................................................. 5.0g

Yeast extract.................................................................................. 5.0g

Meat extract .................................................................................. 2.0g

Glucose ......................................................................................... 2.0g

L-Cysteine·HCl·H2O ..................................................................... 0.3g

NaN3 ........................................................................................... 0.05g

Ethyl Violet ................................................................................. 0.05g

pH 7.4 ± 0.2 at 25°C

Caution: Sodium azide is toxic. Azides also react with metals and

disposal must be highly diluted.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C.

Use: For the isolation and cultivation of Fusobacterium species.