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Vitamin B12 HiVeg Agar

[所属分类:培养基配方] [发布时间:2021-10-11] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose ....................................................................................... 20.0g

K2SO4.......................................................................................... 20.0g

Agar ............................................................................................ 15.0g

Sodium acetate............................................................................ 12.0g

Plant acid hydrolysate, vitamin free ........................................... 10.0g

Soypeptone, vitamin free.............................................................. 5.0g

Na-thioglycollate .......................................................................... 1.7g

K2HPO4......................................................................................... 1.0g

KH2PO4......................................................................................... 1.0g

Polysorbate 80 .............................................................................. 1.0g

Ribonucleic acid ........................................................................... 1.0g

MgSO4 .......................................................................................... 0.4g

L-Cystine....................................................................................... 0.2g

DL-Tryptophan .............................................................................. 0.2g

NaCl............................................................................................ 0.02g

FeSO4.......................................................................................... 0.02g

MnSO4 ........................................................................................ 0.02g

Adenine sulfate ....................................................................... 0.0176g

Guanine hydrochloride ........................................................... 0.0124g

Uracil .......................................................................................... 0.01g

Xanthine (sodium) ...................................................................... 0.01g

Pyridoxal-5-phosphate............................................................... 4.0mg

Pyridoxine hydrochloride .......................................................... 4.0mg

Calcium pantothenate ................................................................ 2.0mg

Niacin......................................................................................... 2.0mg

Riboflavin .................................................................................. 2.0mg

Thiamine hydrochloride............................................................. 2.0mg

Folic acid ................................................................................... 1.0mg

Biotin ..........................................................................................1.0μg

pH 6.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi

Media.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Continue boiling for 2–3 min. Distribute into tubes in 5.0mL

volumes. Add standard solution or test solutions to each tube. Adjust

the volume of each tube to 10.0mL with distilled/deionized water. Au

toclave for 15 min at 15 psi pressure–121°C.

Use: For the microbiological assaying of vitamin B12 using Lactoba

cillus leichmannii as the test organism.

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Vitamin B12 HiVeg Agar