培养基配方

您现在的位置：网站首页 >> 培养基配方

Violet Red Bile Agar, HiVeg

[所属分类:培养基配方] [发布时间:2021-10-11] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Lactose........................................................................................ 10.0g

Plant peptone ................................................................................ 7.0g

NaCl.............................................................................................. 5.0g

Yeast extract.................................................................................. 3.0g

Synthetic detergent No. I .............................................................. 1.5g

Neutral Red................................................................................. 0.03g

Crystal Violet............................................................................. 2.0mg

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi

Media.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Boil to dissolve components

completely. Do not autoclave. Cool to 45°C. Pour into sterile Petri

dishes or leave in tubes.

Use: For the detection of coliform bacteria in water and food.