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Violet Red Bile Agar

[所属分类:培养基配方] [发布时间:2021-10-11] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Lactose ........................................................................................ 10.0g

Glucose ....................................................................................... 10.0g

Pancreatic digest of gelatin ........................................................... 7.0g

NaCl .............................................................................................. 5.0g

Yeast extract.................................................................................. 3.0g

Bile salts........................................................................................ 1.5g

Neutral Red ................................................................................. 0.03g

Crystal Violet .............................................................................2.0mg

pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat while

stirring and bring to boiling. Distribute into tubes or flasks. Autoclave

for 15 min at 15 psi pressure–121°C. Pour immediately into sterile Pe

tri dishes or leave in tubes.

Use: For the isolation and cultivation of members of the Enterobacteri

aceae from brined vegetables. For the enumeration of members of the

Enterobacteriaceae from brined vegetables by the pour plate technique.