培养基配方

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Vibrio costicola Medium

[所属分类:培养基配方] [发布时间:2021-10-9] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl ............................................................................................ 81.0g

MgSO4·7H2O .............................................................................. 19.7g

Agar ............................................................................................ 15.0g

MgCl·H2O................................................................................... 15.0g

Yeast extract................................................................................ 10.0g

Proteose peptone........................................................................... 5.0g

KCl................................................................................................ 2.0g

Glucose ......................................................................................... 1.0g

CaCl2·2H2O ................................................................................ 0.48g

NaHCO3................................................................................... 60.0mg

NaBr......................................................................................... 26.0mg

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Vibrio costicola.