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Venenivibrio stagnispumantis Medium (DSMZ Medium 1146)

[所属分类:培养基配方] [发布时间:2021-10-9] [发布人:网站管理员2] [阅读次数:] [返回]

Venenivibrio stagnispumantis Medium

(DSMZ Medium 1146)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

MgSO4·7H2O................................................................................ 7.0g

Na2S2O3·5H2O .............................................................................. 2.0g

MES ............................................................................................ 1.95g

KCl................................................................................................ 0.5g

MgCl2·6H2O ................................................................................. 0.4g

CaCl2·2H2O .................................................................................. 0.4g

NaOH.......................................................................................... 1.36g

NH4Cl ........................................................................................... 0.2g

KH2PO4....................................................................................... 0.25g

Trace elements solution ...........................................................10.0mL

pH 5.5 ± 0.2 at 25°C

Trace Elements Solution:

Composition per liter:

EDTA ............................................................................................ 5.0g

CoCl2·6H2O ...........................................................................150.0mg

MnCl2·4H2O...........................................................................100.0mg

ZnCl2......................................................................................100.0mg

FeSO4·7H2O...........................................................................100.0mg

AlCl3·6H2O ..............................................................................40.0mg

Na2WO4·2H2O .........................................................................30.0mg

CuCl2·2H2O .............................................................................20.0mg

NiSO4·6H2O.............................................................................20.0mg

NaHSeO3..................................................................................10.0mg

H3BO3 ......................................................................................10.0mg

Na2MoO4·2H2O .......................................................................10.0mg

Preparation of Trace Elements Solution: Add components to dis

tilled/deionized water and bring volume to 1.0L. Adjust pH to 3.0 with

HCl. Mix thoroughly.

Preparation of Medium: Prepare anaerobic distilled/deionized wa

ter by sparging with with 100% CO2. Adjust pH to 5.5. Sparge with

CO2 for 15 min. Add components to the distilled/deionized anaerobic

water and bring volume to 1.0L. Dispense into culture tubes. Stopper

and seal tubes by crimping caps onto stoppers. Autoclave for 20 min at

15 psi pressure–121°C.

Use: For the cultivation of Venenivibrio stagnispumantis.