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Veillonella HiVeg Agar Base with Lactate and Vancomycin

[所属分类:培养基配方] [发布时间:2021-10-9] [发布人:网站管理员2] [阅读次数:] [返回]

Veillonella HiVeg Agar Base

with Lactate and Vancomycin

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Plant hydrolysate........................................................................... 5.0g

Yeast extract.................................................................................. 3.0g

Na-thioglycolate.......................................................................... 0.75g

Basic Fuchsin.............................................................................2.0mg

Sodium lactate (60% solution).................................................21.0mL

Selective supplement solution .................................................10.0mL

pH 7.5± 0.2 at 25°C

Source: This medium, without lactate solution, is available as a pre

mixed powder from HiMedia.

Caution: Basic Fuchsin is a potential carcinogen and care must be

taken to avoid inhalation of the powdered dye and contact with the

skin.

Selective Supplement Solution:

Composition per 10.0mL:

Vancomycin ............................................................................... 7.5mg

Preparation of Selective Supplement Solution: Add vancomy

cin to distilled/deionized water and bring volume to 10.0mL. Mix thor

oughly. Filter sterilize.

Preparation of Medium: Add components, except selective sup

plement solution, to distilled/deionized water and bring volume to

990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave

for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically

add 10.0mL sterile selective supplement solution. Mix thoroughly.

Pour into sterile Petri dishes or aseptically distribute into sterile tubes

or flasks.

Use: For the selective isolation and cultivation of Veillonella species.