培养基配方

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Van Niel’s Medium, Modified

[所属分类:培养基配方] [发布时间:2021-10-9] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract................................................................................ 10.0g

MgSO4 .......................................................................................... 0.1g

EDTA .........................................................................................2.0mg

Trace elements solution ...........................................................10.0mL

K2HPO4 solution........................................................................2.5mL

pH 7.1 ± 0.2 at 25°C

Trace Elements Solution:

Composition per 100.0mL:

CaCl2·2H2O................................................................................... 0.3g

Ferric ammonium citrate............................................................... 0.2g

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 100.0mL. Mix thorough

ly. Filter sterilize.

K2HPO4 Solution:

Composition per 100.0mL:

K2HPO4......................................................................................... 4.0g

Preparation of K2HPO4 Solution: Add K2HPO4 to distilled/de

ionized water and bring volume to 100.0mL. Mix thoroughly. Filter

sterilize.

Preparation of Medium: Add components, except K2HPO4 and

trace elements solution, to distilled/deionized water and bring volume

to 987.5mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 50°C. Aseptically add trace elements and K2HPO4 so

lutions. Mix thoroughly. Distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Rhodobacter sphaeroides.