培养基配方

您现在的位置：网站首页 >> 培养基配方

V24N Medium (DSMZ Medium 88b)

[所属分类:培养基配方] [发布时间:2021-10-9] [发布人:网站管理员2] [阅读次数:] [返回]

V24N Medium

(DSMZ Medium 88b)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Starch, soluble............................................................................... 2.0g

(NH4)2SO4 .................................................................................... 1.3g

Sulfur, powdered........................................................................... 1.0g

Na2S·9H2O.................................................................................... 0.5g

KH2PO4....................................................................................... 0.28g

MgSO4·7H2O.............................................................................. 0.25g

Yeast extract.................................................................................. 0.2g

CaCl2·2H2O ................................................................................ 0.07g

FeCl3·6H2O................................................................................. 0.02g

Na2B4O7·10H2O......................................................................... 4.5mg

MnCl2·4H2O .............................................................................. 1.8mg

Resazurin ................................................................................... 0.4mg

ZnSO4·7H2O ............................................................................ 0.22mg

CuCl2·2H2O ............................................................................. 0.05mg

Na2MoO4·2H2O ...................................................................... 0.03mg

VOSO4·2H2O........................................................................... 0.03mg

CoSO4 ...................................................................................... 0.01mg

pH 6.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust the pH to 6.0

with H2SO4 (25% v/v). Sparge medium with 100% N2 gas. Distribute

anaerobically into rubber-stoppered tubes or bottles that have been pre

sterilized by autoclaving. On 2 successive days heat the medium for 2

hr at 85 °C.

Use: For the growth and maintenance of Thermofilum librum.