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V Agar

[所属分类:培养基配方] [发布时间:2021-10-8] [发布人:网站管理员2] [阅读次数:] [返回]

V Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 13.5g

Pancreatic digest of casein.......................................................... 12.0g

Peptone........................................................................................ 10.0g

Peptic digest of animal tissue........................................................ 5.0g

NaCl .............................................................................................. 5.0g

Beef extract ................................................................................... 3.0g

Yeast extract.................................................................................. 3.0g

Cornstarch..................................................................................... 1.0g

Human blood, anticoagulated ..................................................50.0mL

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a prepared medium from BD Di

agnostic Systems.

Preparation of Medium: Add components, except human blood, to

distilled/deionized water and bring volume to 950.0mL. Mix thorough

ly. Gently heat and bring to boiling. Distribute into tubes or flasks. Au

toclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically

add 50.0mL of human blood. Mix thoroughly. Pour into sterile Petri

dishes or leave in tubes.

Use: For the isolation and differentiation of Gardnerella vaginalis

from clinical specimens. Plates are incubated under an atmosphere

with 3–10% CO2. Gardnerella vaginalis appears as small white colo

nies with diffuse β-hemolysis.