培养基配方

您现在的位置：网站首页 >> 培养基配方

Uric Acid Medium

[所属分类:培养基配方] [发布时间:2021-10-8] [发布人:网站管理员2] [阅读次数:] [返回]

Uric Acid Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Peptone ......................................................................................... 5.0g

NaCl.............................................................................................. 5.0g

Yeast extract.................................................................................. 2.0g

Beef extract................................................................................... 1.0g

Uric acid solution...................................................................150.0mL

Uric Acid Solution:

Composition per 150.0mL:

Uric acid........................................................................................ 6.0g

Preparation of Uric Acid Solution: Add uric acid to distilled/de

ionized water and bring volume to 150.0mL. Mix thoroughly. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.

Preparation of Medium: Add components, except uric acid solu

tion, to distilled/deionized water and bring volume to 750.0mL. Mix

thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 150.0mL of

sterile uric acid solution. Mix thoroughly. Pour into sterile Petri dishes

or distribute into sterile tubes while agitating.

Use: For the cultivation of Bacillus fastidiosus.