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Uric Acid Broth

[所属分类:培养基配方] [发布时间:2021-10-8] [发布人:网站管理员2] [阅读次数:] [返回]

Uric Acid Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Na2HPO4·12H2O........................................................................... 9.0g

NaCl .............................................................................................. 5.0g

KH2PO4......................................................................................... 1.5g

Meat extract .................................................................................. 1.0g

Yeast extract.................................................................................. 1.0g

MgSO4·7H2O................................................................................ 0.2g

MnCl2·4H2O ............................................................................20.0mg

CaCl2..........................................................................................1.2mg

Glucose-uric acid solution .....................................................100.0mL

Glucose-Uric Acid Solution:

Composition per 100.0mL:

Glucose ......................................................................................... 5.0g

Uric acid........................................................................................ 0.4g

Preparation of Glucose-Uric Acid Solution: Add components

to distilled/deionized water and bring volume to 100.0mL. Mix thor

oughly. Filter sterilize.

Preparation of Medium: Add components, except glucose-uric

acid solution, to distilled/deionized water and bring volume to

900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 50°–55°C. Aseptically add 100.0mL of sterile glucose

uric acid solution. Mix thoroughly. Aseptically distribute into sterile

tubes or flasks.

Use: For the cultivation and maintenance of Bacillus species.