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Tryptone Soy Agar with 1% Sodium Chloride

[所属分类:培养基配方] [发布时间:2021-9-27] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Pancreatic digest of casein.......................................................... 15.0g

NaCl ............................................................................................ 15.0g

Pancreatic digest of soybean meal ................................................ 5.0g

Cocarboxylase solution..........................................................100.0mL

pH 7.3 ± 0.2 at 25°C

Cocarboxylase Solution:

Composition per 100.0mL:

Cocarboxylase............................................................................2.0mg

Preparation of Cocarboxylase Solution: Add cocarboxylase to

distilled/deionized water and bring volume to 100.0mL. Mix thorough

ly. Filter sterilize.

Preparation of Medium: Add components, except cocarboxylase

solution, to distilled/deionized water and bring volume to 900.0mL.

Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min

at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 100.0mL

of sterile cocarboxylase solution. Mix thoroughly. Pour into sterile Pe

tri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Haemophilus piscium.