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Tryptone Soy Agar with Horse Blood (LMG Medium 50)

[所属分类:培养基配方] [发布时间:2021-9-27] [发布人:网站管理员2] [阅读次数:] [返回]

Tryptone Soy Agar with Horse Blood

(LMG Medium 50)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Tryptone...................................................................................... 15.0g

Agar ............................................................................................ 15.0g

Soy peptone .................................................................................. 5.0g

NaCl.............................................................................................. 5.0g

Horse blood, sterile defibrinated..............................................50.0mL

pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components, except horse blood, to

distilled/deionized water and bring volume to 950.0mL. Mix thorough

ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL sterile

horse blood. Mix thoroughly. Pour into sterile Petri dishes or distribute

into sterile tubes.

Use: For the cultivation and maintenance of Paenibacillus lentimor

bus.