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Transgrow Medium with Trimethoprim

[所属分类:培养基配方] [发布时间:2021-9-18] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 20.0g

Hemoglobin ................................................................................ 10.0g

Pancreatic digest of casein............................................................ 7.5g

Selected meat peptone .................................................................. 7.5g

NaCl.............................................................................................. 5.0g

K2HPO4......................................................................................... 4.0g

Glucose ......................................................................................... 1.5g

Cornstarch..................................................................................... 1.0g

KH2PO4......................................................................................... 1.0g

Supplement solution ................................................................10.0mL

VCNT inhibitor........................................................................10.0mL

pH 6.7 ± 0.2 at 25°C

Source: This medium is available as a prepared medium from BD Di

agnostic Systems.

Supplement Solution:

Composition per liter:

Glucose ..................................................................................... 100.0g

L-Cysteine·HCl............................................................................ 25.9g

L-Glutamine ................................................................................ 10.0g

L-Cystine ....................................................................................... 1.1g

Adenine......................................................................................... 1.0g

Nicotinamide adenine dinucleotide ............................................ 0.25g

Vitamin B12 ................................................................................... 0.1g

Thiamine pyrophosphate .............................................................. 0.1g

Guanine·HCl ............................................................................... 0.03g

Fe(NO3)3·6H2O........................................................................... 0.02g

p-Aminobenzoic acid................................................................ 0.013g

Thiamine·HCl ............................................................................ 3.0mg

Source: The supplement solution (IsoVitaleX® enrichment) is avail

able from BD Diagnostic Systems. This enrichment may be replaced

by supplement VX from BD Diagnostic Systems.

Preparation of Supplement Solution: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter

sterilize.

VCNT Inhibitor:

Composition per 10.0mL:

Colistin....................................................................................... 7.5mg

Trimethoprim lactate.................................................................. 5.0mg

Vancomycin ............................................................................... 3.0mg

Nystatin.................................................................................. 12,500U

Preparation of VCNT Inhibitor: Add components to distilled/de

ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster

ilize.

Preparation of Medium: Add components, except supplement so

lution and VCNT inhibitor, to distilled/deionized water and bring vol-

ume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C un

der 5–30% CO2. Aseptically add 10.0mL of sterile supplement solu

tion and 10.0mL of sterile VCNT inhibitor. Mix thoroughly.

Aseptically distribute under 5–30% CO2 into sterile screw-capped

tubes.

Use: For the transportation and recovery of pathogenic Neisseria spe

cies.

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Transgrow Medium with Trimethoprim