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TPL Medium (Thioglycolate Potato Liver Medium)

[所属分类:培养基配方] [发布时间:2021-9-17] [发布人:网站管理员2] [阅读次数:] [返回]

TPL Medium

(Thioglycolate Potato Liver Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Potato ........................................................................................ 200.0g

Yeast extract................................................................................ 31.0g

Liver............................................................................................ 25.0g

Glycerol ...................................................................................... 15.0g

Agar ............................................................................................ 15.0g

Meat extract .................................................................................. 5.5g

Glucose ......................................................................................... 7.5g

Peptone ......................................................................................... 2.5g

NaCl.............................................................................................. 2.5g

Sodium thioglycolate.................................................................... 0.5g

Methylene Blue.......................................................................... 1.0mg

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add peeled, sliced potato to approxi

mately 500.0mL of distilled/deionized water. Gently heat and bring to

boiling. Continue boiling for 30 min. Filter through cheesecloth. Cut

up liver into small pieces and add to approximately 150.0mL of dis

tilled/deionized water. Gently heat and bring to boiling. Continue boil

ing for 30 min. Filter through cheesecloth. Add boiled potato solids,

boiled liver solids, and remaining components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Make sure each of the tubes

receives a few pieces of liver. Autoclave for 15 min at 15 psi pressure–

121°C.

Use: For the cultivation and maintenance of Pseudomonas species.