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TPEY Agar (Tellurite Polymyxin Egg Yolk Agar)

[所属分类:培养基配方] [发布时间:2021-9-17] [发布人:网站管理员2] [阅读次数:] [返回]

TPEY Agar

(Tellurite Polymyxin Egg Yolk Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................ 20.0g

Agar ............................................................................................ 15.5g

Pancreatic digest of casein.......................................................... 10.0g

Yeast extract.................................................................................. 5.0g

D-Mannitol .................................................................................... 5.0g

LiCl............................................................................................... 2.0g

Egg yolk emulsion (30% solution) ........................................100.0mL

Chapman tellurite solution.......................................................10.0mL

Polymyxin B solution ................................................................0.4mL

pH 7.1 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Egg Yolk Emulsion (30% Solution):

Composition per 100.0mL:

NaCl.............................................................................................. 0.6g

Egg yolk...................................................................................30.0mL

Preparation of Egg Yolk Emulsion (30% Solution): Add NaCl

and egg yolk to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

Chapman Tellurite Solution:

Composition per 100.0mL:

K2TeO3.......................................................................................... 1.0g

Preparation of Chapman Tellurite Solution: Add K2TeO3 to

distilled/deionized water and bring volume to 100.0mL. Mix thorough

ly. Filter sterilize.

Polymyxin B Solution:

Composition per 100.0mL:

Polymyxin B ................................................................................. 1.0g

Preparation of Polymyxin B Solution: Add polymyxin B to dis

tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Filter sterilize.

Caution: Potassium tellurite is toxic.

Preparation of Medium: Add components—except 30% egg yolk

emulsion, Chapman tellurite solution, and polymyxin B solution—to

distilled/deionized water and bring volume to 890.0mL. Mix thorough

ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C. Aseptically add 100.0mL of sterile

30% egg yolk emulsion, 10.0mL of sterile Chapman tellurite solution,

and 0.4mL of sterile polymyxin B solution. Mix thoroughly. Pour into

sterile Petri dishes or distribute into sterile tubes.

Use: For the recovery of staphylococci from foods and other materials.

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TPEY Agar (Tellurite Polymyxin Egg Yolk Agar)