Petrotoga Medium (ATCC 1881)

[所属分类:培养基配方] [发布时间:2021-7-5] [发布人:网站管理员2] [阅读次数:] [返回]

Petrotoga Medium
(ATCC 1881)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................20.0g
Sodium PIPES (piperazine-N,N′-
bis[2-ethanesulfonic acid]) buffer........................................5.24g
Pancreatic digest of casein............................................................5.0g
Yeast extract..................................................................................2.0g
Resazurin ......................................................................................1.0g
Soluble starch................................................................................1.0g
L -Cysteine·HCl·H 2 O.....................................................................0.5g
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components, except  L -cyste-
ine·HCl·H 2 O, to distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Adjust pH to 7.4. Gently heat and bring to boiling.
Continue boiling for 3 min. Cool to room temperature while sparging
with 100% N 2 . Add  L -cysteine·HCl·H 2 O. Mix thoroughly. Anaerobi-
cally distribute into tubes. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the cultivation of Petrotoga miotherma.