Peptone Meat Extract Soil Extract Agar (PFE Agar)

[所属分类:培养基配方] [发布时间:2021-7-2] [发布人:网站管理员2] [阅读次数:] [返回]

Peptone Meat Extract Soil Extract Agar
(PFE Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................12.0g
Proteose peptone No. 3.................................................................5.0g
Meat extract..................................................................................3.0g
Tap water ...............................................................................850.0mL
Soil extract.............................................................................150.0mL
Glycerol ...................................................................................20.0mL
pH 7.0 ± 0.2 at 25°C
Soil Extract:
Composition per liter:
Garden soil, air dried ................................................................400.0g
Preparation of Soil Extract: Pass 400.0g of air-dried garden soil
through a coarse sieve. Add soil to 960.0mL of tap water. Mix thor-
oughly. Autoclave for 60 min at 15 psi pressure–121°C. Cool to room
temperature. Allow residue to settle. Decant supernatant solution. Fil-
ter through Whatman filter paper. Distribute into bottles in 200.0mL
volumes. Autoclave for 15 min at 15 psi pressure–121°C. Store at room
temperature until clear.
Preparation of Medium: Add components to tap water and bring
volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Dis-
tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Mycobacterium avium,
Mycobacterium gastri, Mycobacterium kansasii, Mycobacterium mari-
num, Mycobacterium scrofulaceum, and Mycobacterium terrae.