Pentachlorophenol Medium

[所属分类:培养基配方] [发布时间:2021-7-1] [发布人:网站管理员2] [阅读次数:] [返回]

Pentachlorophenol Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1007.0mL:

Sodium glutamate.........................................................................4.0g
K 2 HPO 4 .......................................................................................0.65g
NaNO 3 ..........................................................................................0.5g
KH 2 PO 4 .......................................................................................0.19g
MgSO 4 ·7H 2 O................................................................................0.1g
Pentachlorophenol solution........................................................5.0mL
FeSO 4  solution ...........................................................................2.0mL
pH 7.3 ± 0.1 at 25°C
Pentachlorophenol Solution:
Composition per 100.0mL:
Pentachlorophenol ........................................................................1.0g
NaOH (0.5N solution)............................................................100.0mL
Preparation of Pentachlorophenol Solution:  Add pentachloro-
phenol to 100.0mL of NaOH solution. Mix thoroughly. Filter sterilize.
FeSO 4 Solution:
Composition per 100.0mL:
FeSO 4 ............................................................................................2.5g
Preparation of  FeSO 4 Solution:  Add FeSO 4  to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except pentachloro-
phenol solution and FeSO 4 solution, to distilled/deionized water and
bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.3–7.4. Auto-
clave for 15 min at 15 psi pressure–121°C. Aseptically add 2.0mL of
sterile FeSO 4 solution. Mix thoroughly. Aseptically distribute into ster-
ile flasks. Inoculate flasks and place on a shaker at 200 rpm at 25°–
30°C. Monitor growth with a spectrophotometer at 560 nm. When ab-
sorbance at 560 nm (A 560 ) is 0.5, add 5.0mL of sterile pentachlorophe-
nol solution per liter of medium.
Use: For the cultivation of Pseudomonas mendocina.