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培养基配方
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Pentachloronitrobenzene Rose Bengal Yeast Extract Sucrose Agar (PRYES Agar)
[所属分类:培养基配方] [发布时间:2021-7-1] [发布人:网站管理员2] [阅读次数:] [返回]
Pentachloronitrobenzene Rose Bengal Yeast Extract
Sucrose Agar
(PRYES Agar)
Agar............................................................................................20.0g
Yeast extract................................................................................20.0g
Pentachloronitrobenzene (PCNB) ................................................0.1g
Chloramphenicol.........................................................................0.05g
Chlortetracycline·HCl.................................................................0.05g
Rose Bengal..............................................................................0.025g
pH 5.6 ± 0.2 at 25°C
Preparation of Medium: Add components, except chlorampheni-
col and chlortetracycline, to distilled/deionized water and bring vol-
ume to 1.0L. Mix thoroughly. Adjust pH to 5.6 with tartaric acid.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add chloramphenicol and chlortetracycline. Mix thorough-
ly. Pour into sterile Petri dishes.
Use: For the cultivation and differentiation of nephrotoxin-producing
strains of Penicillium viridicatium and related species isolated from
foods. Colonies exhibiting a violet brown pigment on the reverse are
counted as potential ochratoxin- and citrinin-producing strains of Peni-
cillium viridicatium. Colonies exhibiting a yellow reverse and obverse
are counted as potential xanthomegnin- and viomellein-producing
strains of Penicillium viridicatium and Penicillium aurantiogriseum
(Penicillium cyclopium).
Sucrose Agar
(PRYES Agar)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Sucrose......................................................................................150.0gAgar............................................................................................20.0g
Yeast extract................................................................................20.0g
Pentachloronitrobenzene (PCNB) ................................................0.1g
Chloramphenicol.........................................................................0.05g
Chlortetracycline·HCl.................................................................0.05g
Rose Bengal..............................................................................0.025g
pH 5.6 ± 0.2 at 25°C
Preparation of Medium: Add components, except chlorampheni-
col and chlortetracycline, to distilled/deionized water and bring vol-
ume to 1.0L. Mix thoroughly. Adjust pH to 5.6 with tartaric acid.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add chloramphenicol and chlortetracycline. Mix thorough-
ly. Pour into sterile Petri dishes.
Use: For the cultivation and differentiation of nephrotoxin-producing
strains of Penicillium viridicatium and related species isolated from
foods. Colonies exhibiting a violet brown pigment on the reverse are
counted as potential ochratoxin- and citrinin-producing strains of Peni-
cillium viridicatium. Colonies exhibiting a yellow reverse and obverse
are counted as potential xanthomegnin- and viomellein-producing
strains of Penicillium viridicatium and Penicillium aurantiogriseum
(Penicillium cyclopium).
