PDA-LUP

[所属分类:培养基配方] [发布时间:2021-6-30] [发布人:网站管理员2] [阅读次数:] [返回]

PDA-LUP

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Potatoes.....................................................................................500.0g
Glucose.......................................................................................20.0g
Agar............................................................................................15.0g
Lupine stems........................................................................... variable
Preparation of Medium: Slice potatoes with skin. Place 500.0g of
potatoes in 1.0L of distilled/deionized water. Gently heat to 60°C. Al-
low to steep at 60°C for 60 min. Filter through cheesecloth. Add agar
and glucose to filtrate. Bring volume to 1.0L with distilled/deionized
water. Mix thoroughly. Gently heat and bring to boiling. Distribute
6.0mL volumes into tubes. Cut lupine stems into 8.0cm-long pieces.
Add 2–3 lupine stems per tube. Autoclave for 15 min at 15 psi pres-
sure–121°C. Allow tubes to cool in a slanted position.
Use:  For the cultivation of Ceratocystis adiposa, Ceratocystis coerule-
scens, and Ceratocystis fimbriata.