Paracoccus alcaliphilus Medium (DSMZ Medium 772)

[所属分类:培养基配方] [发布时间:2021-6-30] [发布人:网站管理员2] [阅读次数:] [返回]

Paracoccus alcaliphilus Medium
(DSMZ Medium 772)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

(NH 4 ) 2 SO 4 .....................................................................................3.0g
Na 2 HPO 4 .......................................................................................3.0g
Yeast extract..................................................................................2.0g
KH 2 PO 4 .........................................................................................1.4g
MgSO 4 ·7H 2 O................................................................................0.2g
Fe-citrate..................................................................................30.0mg
CaCl 2 ·2H 2 O..............................................................................30.0mg
MnCl 2 ·4H 2 O...............................................................................5.0mg
ZnSO 4 ·7H 2 O..............................................................................5.0mg
CuSO 4 ·2H 2 O..............................................................................0.5mg
Methanol..................................................................................10.0mL
NaHCO 3  solution ....................................................................variable
pH 9.0 ± 0.2 at 25°C
NaHCO 3 Solution:
Composition per 50.0mL :
NaHCO 3 ........................................................................................5.0g
Preparation of NaHCO 3 Solution:  Add NaHCO 3 to distilled/de-
ionized water and bring volume to 50.0mL. Mix thoroughly. Sparge
with 80% N 2 + 20% CO 2 . Filter sterilize.
Preparation of Medium: Add components, except NaHCO 3  solu-
tion and methanol, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Filter sterilize 10.0mL of methanol. Aseptically add the 10.0mL
filter sterilized methanol. Mix thoroughly. Adjust pH to 9.0 using the
sterile NaHCO 3 solution.
Use:  For the cultivation of Paracoccus alcaliphilus.