Pantothenate Assay Medium

[所属分类:培养基配方] [发布时间:2021-6-30] [发布人:网站管理员2] [阅读次数:] [返回]

Pantothenate Assay Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .......................................................................................38.0g
Sodium acetate............................................................................20.0g
Vitamin-free casamino acids.......................................................10.0g
K 2 HPO 4 .........................................................................................3.0g
(NH 4 ) 2 SO 4 .....................................................................................2.0g
NaCl..............................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.4g
L- Tryptophan.................................................................................0.1g
MnSO 4 ·H 2 O..............................................................................0.026g
Xanthine......................................................................................0.02g
Adenine.......................................................................................0.02g
Guanine.......................................................................................0.02g
Uracil ..........................................................................................0.02g
(NH 4 ) 2 SO 4 ·FeSO 4 ·6H 2 O.............................................................0.02g
Niacin.........................................................................................5.0mg
Pyridoxine·HCl..........................................................................4.0mg
Riboflavin ..................................................................................2.0mg
Thiamine·HCl ............................................................................1.0mg
p-Aminobenzoic acid.................................................................1.0mg
Biotin .......................................................................................0.05mg
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Continue boiling for 2–3 min. Distribute into tubes in 5.0mL
volumes. Add standard solution or test solution to each tube. Adjust the
volume of each tube to 10.0mL with distilled/deionized water. Auto-
clave for 15 min at 15 psi pressure–121°C.
Use:  For determination of the pantothenate content of pharmaceutical
products and other materials using Lactobacillus plantarum as the test
organism.