Pagano Levin Agar

[所属分类:培养基配方] [发布时间:2021-6-29] [发布人:网站管理员2] [阅读次数:] [返回]

Pagano Levin Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose.......................................................................................40.0g
Agar............................................................................................15.0g
Peptone .......................................................................................10.0g
Yeast extract..................................................................................1.0g
Neomycin......................................................................................0.5g
2,3,5-Triphenyltetrazolium chloride.............................................0.1g
pH 6.0 ± 0.1 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components, except neomycin and
2,3,5-triphenyltetrazolium chloride, to distilled/deionized water and
bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boil-
ing. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add neomycin and 2,3,5-triphenyltetrazolium chloride.
Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile
tubes. Allow tubes to cool in a slanted position.
Use:  For the isolation, cultivation, and differentiation of Candida spe-
cies. Candida albicans appears as smooth, shiny, cream-light pink col-
onies.