Oxalate Medium

[所属分类:培养基配方] [发布时间:2021-6-29] [发布人:网站管理员2] [阅读次数:] [返回]

Oxalate Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Basal Medium:

Composition per liter:
K 2 HPO 4 .........................................................................................4.4g
KH 2 PO 4 .........................................................................................3.4g
Potasssium oxalate........................................................................2.0g
(NH 4 ) 2 SO 4 .....................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.2g
FeCl 3 .........................................................................................0.015g
Phenol Red (0.4% solution).......................................................5.0mL
Mineral stock solution ...............................................................1.0mL
Mineral Stock Solution:
Composition per liter:
ZnSO 4 ·7H 2 O...............................................................................11.0g
MnSO 4 ·H 2 O..................................................................................5.0g
Na 2 MoO 4 ·2H 2 O ............................................................................2.0g
CoSO 4 .........................................................................................0.05g
H 3 BO 3 .........................................................................................0.05g
CuSO 4 ·5H 2 O..............................................................................7.0mg
Preparation of Mineral Stock Solution: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the isolation and cultivation of oxalate-decomposing Alcali-
genes species.