Oxacillin Resistance Screening Agar Base

[所属分类:培养基配方] [发布时间:2021-6-28] [发布人:网站管理员2] [阅读次数:] [返回]

Oxacillin Resistance Screening Agar Base

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................55.0g
Agar............................................................................................12.5g
Peptic digest of animal tissue ..................................................... 11.8g
Mannitol......................................................................................10.0g
Yeast extract..................................................................................9.0g
LiCl...............................................................................................5.0g
Aniline Blue..................................................................................0.2g
pH 7.2 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Caution: Lithium chloride is harmful. Avoid bodily contact and inha-
lation of vapors. On contact with skin, wash with plenty of water im-
mediately.
Selective Supplement Solution:
Composition per 10.0mL:
Oxacillin.....................................................................................2.0mg
Polymyxin B..........................................................................50,000U
Preparation of Selective Supplement Solution:  Add compo-
nents to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components, except selective sup-
plement solution, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C. Aseptically add selective supplement solution.
Mix thoroughly. Pour into Petri dishes or aseptically distribute into
sterile tubes.
Use: For the screening of oxacillin-resistant microorganisms.