OTI Medium, Modified

[所属分类:培养基配方] [发布时间:2021-6-28] [发布人:网站管理员2] [阅读次数:] [返回]

OTI Medium, Modified

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1100.0mL:

Beef heart, solids from infusion................................................100.0g
NaCl..............................................................................................6.0g
Polypeptone™...............................................................................5.0g
Yeast extract..................................................................................5.0g
K 2 HPO 4 .........................................................................................2.0g
Tryptose ........................................................................................2.0g
Agar ..............................................................................................1.6g
Pectin ............................................................................................0.8g
Glucose .........................................................................................0.8g
Starch ............................................................................................0.8g
Sucrose..........................................................................................0.8g
Maltose..........................................................................................0.8g
Sodium pyruvate...........................................................................0.8g
Ribose ...........................................................................................0.8g
L -Cysteine·HCl·H 2 O ...................................................................0.68g
MgSO 4 ·7H 2 O................................................................................0.1g
Rumen fluid, clarified...........................................................500.0 mL
Rabbit serum, inactivated ........................................................50.0mL
Thiamine pyrophosphate solution............................................50.0mL
pH 7.0 ± 0.2 at 25°C
Thiamine Pyrophosphate Solution:
Composition per 50.0mL:
Thiamine pyrophosphate ...........................................................7.5mg
Preparation of Thiamine Pyrophosphate Solution: Add thia-
mine pyrophosphate to distilled/deionized water and bring volume to
50.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Prepare and dispense medium under O 2 -
free 100% N 2 . Add components, except rabbit serum and thiamine py-
rophosphate solution, to distilled/deionized water and bring volume to
1.0L. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to
7.0. Anaerobically distribute into tubes in 10.0mL volumes. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add 0.5 mL of sterile rabbit solution and 0.5mL of sterile thiamine py-
rophosphate solution to each tube. Mix thoroughly.
Use:  For the cultivation and maintenance of Treponema socranskii.