OS Solid Medium (DSMZ Medium 887)

[所属分类:培养基配方] [发布时间:2021-6-28] [发布人:网站管理员2] [阅读次数:] [返回]

OS Solid Medium
(DSMZ Medium 887)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Gelrite.........................................................................................15.0g
NaHCO 3 ........................................................................................1.0g
Na 2 S 2 O 3 ·5H 2 O..............................................................................1.0g
NaCl.......................................................................................256.0mg
Na 2 SO 4 .....................................................................................23.0mg
KCl...........................................................................................15.0mg
H 3 BO 3 ......................................................................................10.3mg
KH 2 PO 4 ......................................................................................1.7mg
CaCl 2 ·2H 2 O ...............................................................................0.8mg
NaNO 3 .......................................................................................0.3mg
FeCl 3 ·6H 2 O................................................................................0.1mg
MnSO 4 ·4H 2 O...........................................................................0.06mg
Trace elements solution...........................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
MgSO 4 ·7H 2 O................................................................................3.0g
Nitrilotriacetic acid.......................................................................1.5g
NaCl..............................................................................................1.0g
MnSO 4 ·2H 2 O................................................................................0.5g
CoSO 4 ·7H 2 O...............................................................................0.18g
ZnSO 4 ·7H 2 O...............................................................................0.18g
CaCl 2 ·2H 2 O ..................................................................................0.1g
FeSO 4 ·7H 2 O..................................................................................0.1g
NiCl 2 ·6H 2 O...............................................................................0.025g
KAl(SO 4 ) 2 ·12H 2 O.......................................................................0.02g
H 3 BO 3 .........................................................................................0.01g
Na 2 MoO 4 ·4H 2 O..........................................................................0.01g
CuSO 4 ·5H 2 O...............................................................................0.01g
Na 2 SeO 3 ·5H 2 O...........................................................................0.3mg
Preparation of Trace Elements Solution: Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH
to 6.5 with KOH. Add remaining components. Add distilled/deionized
water to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0 with
Na 2 SO 4 . Autoclave for 15 min at 15 psi pressure–121°C. Pour into Pe-
tri dishes. Incubate under atmosphere of 94% N 2  + 3% H 2 + 3% O 2 .
Use: For the cultivation and maintenance of Thermocrinis ruber.