ONR7a Medium (DSMZ Medium 950)

[所属分类:培养基配方] [发布时间:2021-6-28] [发布人:网站管理员2] [阅读次数:] [返回]

ONR7a Medium
(DSMZ Medium 950)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Solution 1...............................................................................800.0mL
Solution 2...............................................................................150.0mL
Solution 3.................................................................................50.0mL
Solution 1:
Composition per 800.0mL:
NaCl..........................................................................................22.79g
Na 2 SO 4 ........................................................................................3.98g
TAPSO..........................................................................................1.3g
KCl..............................................................................................0.72g
NH 4 Cl .........................................................................................0.27g
NaBr.........................................................................................83.0mg
NaHCO 3 ...................................................................................31.0mg
H 3 BO 3 ......................................................................................27.0mg
NaF...........................................................................................2.60mg
Na 2 HPO 4 ·7H 2 O........................................................................89.0mg
pH 7.6 ± 0.2 at 25°C
Preparation of Solution 1:  Add components to distilled/deionized
water and bring volume to 800.0mL. Mix thoroughly. Adjust pH to 7.6.
with NaOH. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
25°C.
Solution 2:
Composition per 150.0mL:
MgCl 2 ·6H 2 O..............................................................................11.18g
CaCl 2 ·2H 2 O.................................................................................1.46g
SrCl 2 ·6H 2 O...............................................................................24.0mg
Preparation of Solution 2:  Add components to distilled/deionized
water and bring volume to 150.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 25°C.
Solution 3:
Composition per 50.0mL:
FeCl 2 ·4H 2 O................................................................................2.0mg
Preparation of Solution 3:  Add FeCl 2 ·4H 2 O to distilled/deionized
water and bring volume to 50.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 25°C.
Preparation of Medium: Aseptically combine 800.0mL sterile so-
lution 1, 150.0mL sterile solution 2, and 50.0mL sterile solution 3. Mix
thoroughly. Distribute into sterile tubes or flasks.
Use: For the cultivation of Oleiphilus messinensis.