HiCrome™ MS.O157 Agar (MS.O157 Agar HiCrome™)

[所属分类:培养基配方] [发布时间:2021-4-8] [发布人: ] [阅读次数:] [返回]

HiCrome™ MS.O157 Agar

(MS.O157 Agar HiCrome™)

培养基配方

Composition per liter:
Agar............................................................................................12.0g
Peptone, special ..........................................................................10.0g
Sorbitol .........................................................................................4.0g
Bile salt mixture............................................................................1.0g
Chromogenic mixture...............................................................0.731g
pH 6.8 ± 0.2 at 25°C

Source: This medium is available from Fluka, Sigma-Aldrich. 

Preparation of Medium:  Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Do not autoclave. Cool to 50°C. Pour into sterile Petri dishes.

Use: For the simultaneous detection of Escherichia coli, Escherichia coli O157:H7 and coliforms in water and food samples. Escherichia coli O157:H7 gives colorless colonies because of non-fermentation of sorbitol and absence of β-glucuronidase activity, whereas other strains of Escherichia coli having β-glucuronidase activity and fermenting sorbitol appear as steel blue colored colonies. Some non Escherichia coli O157:H7 may have some colony color.