HiCrome™ MeReSa Agar with Oxacillin

[所属分类:培养基配方] [发布时间:2021-4-8] [发布人: ] [阅读次数:] [返回]

HiCrome™ MeReSa Agar with Oxacillin

培养基配方

Composition per liter:
NaCl........................................................................................... 40.0g
Agar ............................................................................................15.0g
Casein enzymic hydrolysate ......................................................13.0 g
Chromogenic mixture..................................................................5.3 g
Sodium pyruvate..........................................................................5.0 g
Beef extract..................................................................................2.5 g
Yeast extract.................................................................................2.5 g
MRSA selective supplement....................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Source: This medium, without MRSA supplement, is available as a premixed powder from HiMedia.
MRSA Selective Supplement:
Composition per 10.0mL:
Oxacillin.....................................................................................2.0mg
Preparation of MRSA Selective Supplement: Add oxacillin to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except MRSA supplement, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add 10.0mL MRSA supplement. Pour into sterile Petri dishes or leave in tubes.
Use: For the isolation and cultivation of methicillin-resistant Staphylococcus aureus (MRSA).