HiCrome™ M-CP Agar Base (M-CP HiCrome™ Agar Base) (Membrane Clostridium perfringens HiCrome™ Agar Base)

[所属分类:培养基配方] [发布时间:2021-4-8] [发布人: ] [阅读次数:] [返回]

HiCrome™ M-CP Agar Base
(M-CP HiCrome™ Agar Base)
(Membrane Clostridium perfringens

HiCrome™ Agar Base)

培养基配方

Composition per liter:
Tryptose......................................................................................30.0g
Yeast extract................................................................................20.0g
Agar............................................................................................15.0g
Sucrose..........................................................................................5.0g
L -Cysteine·HCl·H 2 O.....................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.1g
FeCl 3 ·6H 2 O.................................................................................0.09g
Indoxyl-β- D -glucoside................................................................0.06g
Bromcresol Purple......................................................................0.04g
pH 7.6 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from HiMedia.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes.
Use: For the detection of Clostridium perfringens. Recommended by the Directive of the Council of the European Union 98/83/EC for iso- lation and enumeration of C. perfringens from water samples using the membrane filtration technique.