Fletcher’s Semisolid Medium

[所属分类:培养基配方] [发布时间:2020-7-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Fletcher’s Semisolid Medium

培养基配方
Composition per 2120.0mL:
Agar.........................................................................1.5g
NaCl.........................................................................0.5g
Peptone .....................................................................0.3g
Beef extract.................................................................0.2g
Rabbit serum..............................................................240.0mL
pH 7.9 ± 0.1 at 25°C
Preparation of Medium: Add components, except rabbit serum, to
distilled/deionized water and bring volume to 1880.0mL. Mix thor-
oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°–55°C. Aseptically add 240.0mL of sterile
rabbit serum. Mix thoroughly. Aseptically distribute into sterile tubes
or flasks.
Use: For the isolation, cultivation, and maintenance of cultures of Lep-
tospira species.