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Fe(III) Lactate Nutrient Agar

[所属分类:培养基配方] [发布时间:2020-7-14] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Fe(III) Lactate Nutrient Agar

培养基配方
Composition per liter:
Agar........................................................................15.0g
Peptone .....................................................................5.0g
NaCl.........................................................................5.0g
Yeast extract................................................................2.0g
Beef extract.................................................................1.0g
Fe(III)-lactate solution...................................................25.0mL
pH 7.2 ± 0.2 at 25°C
Fe(III)-Lactate Solution:
Composition per 30.0mL:
FeCl3 ·6H2O solution.....................................................20.0mL
Sodium lactate solution...................................................10.0mL
Preparation of Fe(III)-Lactate Solution: Aseptically combine
the component solutions. Mix thoroughly.
FeCl3 ·6H2O Solution:
Composition per 100.0mL:
FeCl3 ·6H2O................................................................5.0g
Preparation of FeCl3 ·6H2O Solution: Add FeCl3 ·6H2O to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize.
Sodium Lactate Solution
Composition per 100.0mL:
Sodium lactate .............................................................5.0g
Preparation of Sodium Lactate Solution: Add sodium lactate to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except Fe(III)-lactate
solution, to distilled/deionized water and bring volume to 975.0L. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 25.0mL of
filter-sterilized Fe(III)-lactate solution. Mix thoroughly. Pour into ster-
ile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Shewanella putrefaciens.

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