- 定做培养基/定制培养基
- 颗粒培养基
- 标准菌株生化鉴定试剂盒
- 预灌装即用型成品培养基
- 2025年版中国药典
- 促销/特价商品
- 院感/疾控/体外诊断/采样管
- 样品采集与处理(均质)产品
- 按标准检索培养基
- 模拟灌装用培养基
- 干燥粉末培养基
- 培养基添加剂/补充剂
- 生化反应鉴定管
- 染色液等配套产品
- 对照培养基/标准品
- 实验耗材与器具
- 生化试剂/化学试剂
- 菌种鉴定服务
Fe(III) Lactate Nutrient Agar
山东拓普生物工程有限公司
Shandong Tuopu Biol-Engineering Co.,Ltd
Fe(III) Lactate Nutrient Agar
培养基配方
Composition per liter:
Agar........................................................................15.0g
Peptone .....................................................................5.0g
NaCl.........................................................................5.0g
Yeast extract................................................................2.0g
Beef extract.................................................................1.0g
Fe(III)-lactate solution...................................................25.0mL
pH 7.2 ± 0.2 at 25°C
Fe(III)-Lactate Solution:
Composition per 30.0mL:
FeCl3 ·6H2O solution.....................................................20.0mL
Sodium lactate solution...................................................10.0mL
Preparation of Fe(III)-Lactate Solution: Aseptically combine
the component solutions. Mix thoroughly.
FeCl3 ·6H2O Solution:
Composition per 100.0mL:
FeCl3 ·6H2O................................................................5.0g
Preparation of FeCl3 ·6H2O Solution: Add FeCl3 ·6H2O to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize.
Sodium Lactate Solution
Composition per 100.0mL:
Sodium lactate .............................................................5.0g
Preparation of Sodium Lactate Solution: Add sodium lactate to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except Fe(III)-lactate
solution, to distilled/deionized water and bring volume to 975.0L. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 25.0mL of
filter-sterilized Fe(III)-lactate solution. Mix thoroughly. Pour into ster-
ile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Shewanella putrefaciens.