Esculin Agar

[所属分类:培养基配方] [发布时间:2020-7-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Esculin Agar

培养基配方
Composition per liter:
Agar........................................................................15.0g
Pancreatic digest of casein.................................................13.0g
NaCl.........................................................................5.0g
Yeast extract................................................................5.0g
Heart muscle, solids from infusion...........................................2.0g
Esculin .....................................................................1.0g
Ferric citrate...............................................................0.5g
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into screw-capped tubes in 3.0mL volumes. Au-
toclave for 15 min at 15 psi pressure–121°C. Allow tubes to cool in a
slanted position.
Use:  For the cultivation and differentiation of bacteria based on their
ability to hydrolyze esculin and produce H 2 S. Bacteria that hydrolyze
esculin appear as colonies surrounded by a reddish-brown to dark
brown zone. Bacteria that produce H 2 S appear as black colonies.