Erythromicrobium roseococcus Medium (DSMZ Medium 767)

[所属分类:培养基配方] [发布时间:2020-7-8] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Erythromicrobium roseococcus Medium
(DSMZ Medium 767)

培养基配方
Composition per 1001.0mL:
Na-acetate...................................................................1.0g
Yeast extract................................................................1.0g
Peptone .....................................................................1.0g
MgSO4 ·7H2O.................................................................0.5g
NH4Cl .......................................................................0.3g
K2HPO4 ......................................................................0.3g
KCl..........................................................................0.3g
CaCl2 ·2H2O ...............................................................0.05g
Trace elements solution SL-6................................................1.0mL
Vitamin B 12 solution.......................................................1.0mL
pH 7.5–7.8 at 25°C
Vitamin B 12 Solution:
Composition per 100.0mL:
Vitamin B 12 ...............................................................2.0mg
Preparation of Vitamin B 12 Solution:  Add vitamin B 12 to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize.
Trace Elements Solution SL-6:
Composition per liter:
MnCl2 ·4H2O .............................................................0.5g
H3BO3 ....................................................................0.3g
CoCl2 ·6H2O..............................................................0.2g
ZnSO4 ·7H2O..............................................................0.1g
Na2MoO4 ·2H2O...........................................................0.03g
NiCl2 ·6H2O.............................................................0.02g
CuCl2 ·2H2O.............................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except vitamin solution,
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 7.5–7.8. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to room temperature. Aseptically add 10mL vitamin solution. Mix
thoroughly. Aseptically distribute into sterile tubes or bottles.
Use:  For the cultivation of Erythrobacter litoralis, Erythromicrobium
ramosum, and Roseococcus thiosulfatophilus.