Enterobacteria Enrichment Broth, Mossel

[所属分类:培养基配方] [发布时间:2020-7-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Enterobacteria Enrichment Broth, Mossel

培养基配方
Composition per liter:
Pancreatic digest of gelatin..........................................10.0g
Na2HPO4 , anhydrous ...................................................6.4g
Glucose, anhydrous.....................................................4.5g
Bile salts (sodium desoxycholate, sodium lauryl sulphate,
sodium citrate)......................................................3.055g
KH2PO4 ................................................................2.0g
Brilliant Green .....................................................0.015g
pH 7.2 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring and bring to boiling. Maintain at 100°C for 30 min, typically
using flowing steam. Do not autoclave. Cool rapidly in cold running
water. MixAseptically distribute into tubes or flasks.
Use: For the enrichment of bile-tolerant Gram-negative bacteria in the
microbiological examination of pharmaceutical products.