Enrichment Broth, pH 7.3

[所属分类:培养基配方] [发布时间:2020-7-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Enrichment Broth, pH 7.3

培养基配方
Composition per liter:
Pancreatic digest of casein.................................................17.0g
Yeast extract................................................................6.0g
NaCl.........................................................................5.0g
Papaic digest of soybean meal................................................3.0g
Glucose......................................................................2.5g
K2HPO4 ......................................................................2.5g
Nalidixic acid solution.....................................................8.0mL
Cycloheximide solution......................................................5.1mL
Acriflavin·HCl solution ...................................................2.0mL
pH 7.3 ± 0.2 at 25°C
Cycloheximide Solution:
Composition per 10.0mL:
Cycloheximide................................................................0.1g
Ethanol, absolute............................................................4.0mL
Preparation of Cycloheximide Solution: Add components to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Nalidixic Acid Solution:
Composition per 10.0mL:
Nalidixic acid..............................................................0.05g
Preparation of Nalidixic Acid Solution: Add nalidixic acid to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Acriflavin·HCl Solution:
Composition per 10.0mL:
Acriflavin·HCl..............................................................0.05g
Preparation of Acriflavin·HCl Solution: Add acriflavin·HCl to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components—except cyclohexim-
ide solution, nalidixic acid solution, and acriflavin·HCl solution—to
distilled/deionized water and bring volume to 984.9mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add 5.1mL of cyclo-
heximide solution, 8.0mL of nalidixic acid solution, and 2.0mL of
acriflavin·HCl solution. Mix thoroughly. Aseptically distribute into
sterile tubes.
Use: For the isolation, cultivation, and enrichment of a variety of
microorganisms from milk and dairy products.