Enriched Thioglycollate HiVeg Broth

[所属分类:培养基配方] [发布时间:2020-7-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Enriched Thioglycollate HiVeg Broth

培养基配方
Composition per liter:
Plant hydrolysate .......................................................17.0g
Glucose...................................................................6.0g
Papaic digest of soybean meal.............................................3.0g
NaCl......................................................................2.5g
Agar......................................................................0.7g
Na-thioglycollate ........................................................0.5g
L -Cystine...............................................................0.25g
Na2SO3 ...................................................................0.1g
Fe4(P2O7)3 ·H2O .......................................................0.005g
Vitamin K 1 ............................................................0.001g
pH 7.1 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Prepare
freshly or boil and cool the medium just before use.
Use: For the cultivation of both aerobic and anaerobic organisms. For
the performance of sterility tests of turbid or viscous specimens.