Desulfovibrio Medium (DSMZ Medium 63b)

[所属分类:培养基配方] [发布时间:2020-6-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfovibrio Medium
(DSMZ Medium 63b)

培养基配方
Composition per liter:
Soultion A..................................................................980.0mL
Solution B...................................................................10.0mL
Solution C...................................................................10.0mL
Solution A:
Composition per 980.0mL:
MgSO4 ·7H2O..................................................................2.0g
DL -Na-lactate................................................................2.0g
Yeast extract.................................................................1.0g
NH4Cl ........................................................................1.0g
Na2SO4 .......................................................................1.0g
K2HPO4 .......................................................................0.5g
CaCl2 ·2H2O ..................................................................0.1g
Resazurin ....................................................................1.0mg
Seawater.....................................................................980.0mL
pH 7.8 ± 0.2 at 25°C
Preparation of Solution A: Add components to filtered aged sea-
water and bring volume to 980.0mL. Mix thoroughly.
Solution B:
Composition per 10.0mL:
FeSO4 ·7H2O..................................................................0.5g
Preparation of Solution B: Add FeSO4 ·7H2O to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly.
Solution C:
Composition per 10.0mL:
Na-thioglycolate ............................................................0.1g
Ascorbic acid................................................................0.1g
Preparation of Solution C: Add components to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly.
Preparation of Medium: Bring solution A to a boil for a few min-
utes. Cool to room temperature while gassing with oxygen-free N2  gas.
Add solutions B and C. Adjust pH to 7.8 with NaOH. Immediately dis-
tribute under N2  into anaerobic tubes. During distribution continuously
swirl the medium to keep the grey precipitate suspended. Autoclave for
15 min at 15 psi pressure–121°C.
Use: For the cultivation of Desulfovibrio indonesiensis.