Desulfovibrio halophilus Medium

[所属分类:培养基配方] [发布时间:2020-6-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfovibrio halophilus Medium

培养基配方
Composition per liter:
NaCl.........................................................................70.0g
MgCl2 ·6H2O .................................................................3.0g
Na2SO4 .......................................................................3.0g
NaHCO3 .......................................................................2.5g
KCl...........................................................................0.3g
NH4Cl ........................................................................0.3g
KH2PO4 .......................................................................0.2g
Na2S·9H2O....................................................................0.2g
CaCl2 ·2H2O.................................................................0.15g
Wolfe’s vitamin solution...................................................10.0mL
Sodium lactate...............................................................3.7mL
Trace elements solution SL-10 ...............................................1.0mL
pH 6.9–7.1 at 25°C
Trace Elements Solution SL-10:
Composition per liter:
FeCl2 ·4H2O.................................................................1.5g
CoCl2 ·6H2O .............................................................190.0mg
MnCl2 ·4H2O..............................................................100.0mg
ZnCl2 .....................................................................70.0mg
Na2MoO4 ·2H2O ............................................................36.0mg
NiCl2 ·6H2O...............................................................24.0mg
H3BO3 ......................................................................6.0mg
CuCl2 ·2H2O ...............................................................2.0mg
HCl (25% solution).........................................................10.0mL
Preparation of Trace Elements Solution SL-10: Add FeCl2 ·4H2O
to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized water
and bring volume to 1.0L. Add remaining components. Mix thoroughly.
Wolfe’s Vitamin Solution:
Composition per liter:
Pyridoxine·HCl............................................................10.0mg
p-Aminobenzoic acid........................................................5.0mg
Lipoic acid ...............................................................5.0mg
Nicotinic acid.............................................................5.0mg
Riboflavin ................................................................5.0mg
Thiamine·HCl .............................................................5.0mg
Calcium  DL -pantothenate..................................................5.0mg
Biotin ....................................................................2.0mg
Folic acid.................................................................2.0mg
Vitamin B 12 ..............................................................0.1mg
Preparation of Wolfe’s Vitamin Solution:  Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Prepare and dispense medium under 90%
N2 + 10% CO2 . Add components, except NaHCO3 and Na2S·9H2O, to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat and bring to boiling. Continue boiling for 3 min. Cool to
room temperature while sparging with 90% N2 + 10% CO2 . Add
NaHCO3  and Na2S·9H2O. Mix thoroughly. Anaerobically distribute
into tubes. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Desulfovibrio halophilus.