Desulfovibrio gigas Medium

[所属分类:培养基配方] [发布时间:2020-6-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfovibrio gigas Medium

培养基配方
Composition per 1001.0mL:
Solution A................................................................950.0mL
Solution B.................................................................40.0mL
Solution C..................................................................6.0mL
Solution D (Vitamin solution)...............................................5.0mL
pH 7.2 ± 0.2 at 25°C
Solution A:
Composition per 950.0mL:
Na2SO4 ......................................................................2.0g
Sodium ( L )-lactate ........................................................2.0g
KH2PO4 ......................................................................1.0g
NH4Cl .......................................................................0.5g
MgSO4 ·7H2O.................................................................0.4g
CaCl2 ·2H2O ................................................................0.1g
H2SO4 (1M solution).........................................................1.0mL
Trace elements solution SL-6................................................1.0mL
Trace Elements Solution SL-6:
Composition per liter:
MnCl2 ·4H2O ...............................................................0.5g
H3BO3 ......................................................................0.3g
CoCl2 ·6H2O................................................................0.2g
ZnSO4 ·7H2O................................................................0.1g
Na2MoO4 ·2H2O.............................................................0.03g
NiCl2 ·6H2O...............................................................0.02g
CuCl2 ·2H2O...............................................................0.01g
Preparation of Trace Elements Solution SL-6: Add compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Gas under 100% N2 . Autoclave for 15 min at 15 psi pressure–
121°C.
Preparation of Solution A:  Add components to distilled/deionized
water and bring volume to 950.0mL. Mix thoroughly. Gently heat and
bring to boiling. Continue boiling for 3–4 min. Allow to cool to room
temperature while gassing under 80% N2 + 20% CO2 . Seal the flask
under 80% N2 + 20% CO2 . Autoclave for 15 min at 15 psi pressure–
121°C.
Solution B:
Composition per 40.0mL:
NaHCO3 ....................................................................2.0g
Preparation of Solution B:  Add NaHCO3 to distilled/deionized
water and bring volume to 40.0mL. Mix thoroughly. Filter sterilize.
Gas under 80% N2 + 20% CO2 .
Solution C:
Composition per 10.0mL:
Na2S·9H2O..................................................................0.5g
Preparation of Solution C:  Add Na2S·9H2O to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Gas under 100%
N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Solution D (Vitamin Solution):
Composition per liter:
Pyridoxine·HCl.............................................................62.5g
Nicotinic acid.............................................................25.0mg
p-Aminobenzoic acid........................................................12.5mg
Thiamine·HCl .............................................................12.5mg
Calcium  DL -pantothenate..................................................6.5mg
Biotin.....................................................................2.5mg
Preparation of Solution D (Vitamin Solution):  Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gas under 100% N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Aseptically and anaerobically combine
950.0mL of sterile solution A with 40.0mL of sterile solution B, 6.0mL
of sterile solution C, and 5.0mL of sterile solution D. Adjust pH to 7.2.
Mix thoroughly. Anaerobically distribute into sterile tubes or flasks un-
der 80% N2 + 20% CO2
Use:  For the cultivation and maintenance of Desulfovibrio gigas.