Defined Medium with Povidone Iodine

[所属分类:培养基配方] [发布时间:2020-6-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Defined Medium with Povidone Iodine

培养基配方
Composition per 1025.0mL:
Basal solution..................................................................1.0L
Solution B....................................................................10.0mL
Solution C....................................................................10.0mL
Solution A.....................................................................5.0mL
Basal Solution:
Composition per liter:
Agar...........................................................................20.0g
Na2HPO4 ........................................................................4.8g
KH2PO4 .........................................................................4.4g
NH4Cl ..........................................................................1.0g
MgSO4 ·7H2O....................................................................0.5g
Preparation of Basal Solution: Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly. Gently heat
and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 45°–50°C.
Solution A:
Composition per 100.0mL:
Ferric ammonium citrate........................................................1.0g
CaCl2 ·2H2O ..................................................................0.1g
Preparation of Solution A: Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Solution B:
Composition per 100.0mL:
D- Glucose......................................................................0.0g
Preparation of Solution B: Add glucose to distilled/deionized wa-
ter and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Solution C:
Composition per 100.0mL:
Povidone-iodine.................................................................0.1g
Preparation of Solution C: Add povidone-iodine to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: To 1.0L of cooled, sterile basal solution,
aseptically add 5.0mL of sterile solution A, 10.0mL of sterile solution
B, and 10.0mL of sterile solution C. Mix thoroughly. Pour into sterile
Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Pseudomonas aeruginosa
and Pseudomonas cepacia.