Cornstarch Soluble Medium (CSSM)

[所属分类:培养基配方] [发布时间:2020-5-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cornstarch Soluble Medium
(CSSM)

培养基配方
Composition per liter:
Cornstarch...................................................................42.0g
n-Butanol ...................................................................18.0g
Yeast extract................................................................10.0g
Asparagine·H2O...............................................................2.0g
(NH4)2SO4 ....................................................................2.0g
NaCl..........................................................................1.0g
KH2PO4 ......................................................................0.75g
K2HPO4 ......................................................................0.75g
L -Cysteine·HCl·H2O ........................................................0.5g
MgSO4 .......................................................................0.02g
FeSO4 ·7H2O..................................................................0.01g
MnSO4 ·H2O...................................................................0.01g
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat until
boiling. Boil and cool under 80% N2 + 10% H2 + 10% CO2 . Distribute
anaerobically into tubes under the same gas mixture. Cap with butyl
rubber stoppers. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation and maintenance of Clostridium thermoam-
ylolyticum.