Cornmeal Yeast Extract Seawater Agar (ATCC Medium 2422)

[所属分类:培养基配方] [发布时间:2020-5-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cornmeal Yeast Extract Seawater Agar
(ATCC Medium 2422)

培养基配方

Composition per liter:
Instant ocean.................................................................17.5g
Agar .........................................................................15.0g
Yeast extract..................................................................1.0g
Cornmeal infusion...........................................................400.0mL
pH 7.2–7.5 at 25°C
Cornmeal Infusion:
Composition per liter:
Yellow cornmeal..............................................................50.0g
Preparation of Cornmeal Infusion: Add cornmeal to distilled/deion-
ized water and bring volume to 1.0L. Gently heat and bring to boiling.
Simmer for 10 minutes. Filter through cheesecloth. Return volume to
1.0 liter.
Preparation of Medium: Add instant ocean, agar, and yeast extract
to 400.0mL cornmeal infusion and bring volume to 1.0L with distilled/
deionized water. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Pour into sterile Petri dishes or distribute
into sterile tubes.
Use:  For the cultivation and maintenance of fungi.