Cornmeal Agar (CMA)

[所属分类:培养基配方] [发布时间:2020-5-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cornmeal Agar
(CMA)

培养基配方
Composition per liter:
Agar ......................................................................20.0g
Cornmeal polenta...........................................................15.0g
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add cornmeal polenta to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly. Gently heat and
bring to boiling. Continue boiling for 30 min. Filter through Whatman
#1 filter paper. Add agar to filtrate. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 10 min at 15 psi pressure–
121°C. Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation and maintenance of many filamentous fungi.