Coprinus Medium

[所属分类:培养基配方] [发布时间:2020-5-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Coprinus Medium

培养基配方
Composition per 1026.0mL:
Agar .....................................................................20.0g
Glucose ..................................................................20.0g
Asparagine.................................................................2.0g
Pancreatic digest of casein...............................................0.75g
Yeast extract.............................................................0.75g
Malt extract..............................................................0.60g
Salt solution ...........................................................25.0mL
Thiamine solution.........................................................1.0mL
pH 6.8 ± 0.2 at 25°C
Salt Solution:
Composition per 500.0mL:
Na2HPO4 ..................................................................45.0g
KH2PO4 ...................................................................20.0g
Ammonium tartrate.........................................................10.0g
Na2SO4 ·10H2O.............................................................5.6g
Preparation of Salt Solution: Add components to distilled/deion-
ized water and bring volume to 500.0mL. Mix thoroughly. Filter ster-
ilize.
Thiamine Solution:
Composition per 100.0mL:
Thiamine.................................................................10.0mg
Preparation of Thiamine Solution: Add thiamine to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except salt solution
and thiamine solution, to distilled/deionized water and bring volume to
1.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for
15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add
25.0mL of sterile salt solution and 1.0mL of sterile thiamine solution.
Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile
tubes.
Use:  For the cultivation and maintenance of Coprinus cinereus, Den-
drophoma obscurans, and Trichophyton violaceum.